FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763331

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763331
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4202533
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG13750.048.189893
CCGAGTA15850.045.6612852
CCCGAGT19400.045.3679851
GAGTAGC36750.043.2070664
AGTAGCT60400.042.29925
GCTGGGA119850.041.197869
CGTACCG1202.0192601E-839.7964553
GTAGCTG64250.039.5356946
TTGGGAG85550.037.688946
TAGCTGG72400.037.632377
CGGAGGT16250.035.8762747
TACTCGG12150.034.3920023
GGGAGGC104650.034.1640938
GGAGGCT85650.033.4775969
CTACTCG12350.032.933212
TGGGAGG112250.031.5329977
CTCGCGT1551.9174331E-730.8097924
AAGTGCT82250.030.699395
CCCAAAG102700.030.548841
GCTACTC29300.029.8345221
Produced by FastQC (version 0.11.5)