FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763332

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763332
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4202533
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG13400.050.1876753
CTACTCG13750.049.775842
TACTCGG14950.048.5670933
CCGAGTA14450.048.1882862
GCTACTC35500.047.4813271
TTGGGAG101400.046.6447536
GAGTAGC36150.044.28614
GGAGGCT99250.043.698919
GCTGGGA120150.043.425969
AGTAGCT59100.043.2975275
CCCGAGT18850.042.9726641
CGGAGGT21050.042.960087
GGGAGGC127600.040.750638
GTAGCTG61750.040.0802086
CTACTTG32950.039.375362
TCGGGAG20750.037.5601046
GCACTTT86550.037.230161
CTCGGGA18950.037.055695
TGGGAGG134450.036.9044577
TAGCTGG71250.036.740197
Produced by FastQC (version 0.11.5)