FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763333

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763333
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3417385
Sequences flagged as poor quality0
Sequence length35-351
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTCACG5956.8553254E-6114.56664300-309
CGCGTAG1900.0088830689.693634300-309
CGAGTAG24950.076.205133
AGTAGCT95750.075.620465
AAATTCG4500.001881054573.00528340-345
GTAGCTG101200.071.646576
GAGTAGC62250.070.3974
CCGAGTA27200.069.254362
GTCGTAA2350.001532135567.508354290-299
GCTGGGA205150.065.969389
TTGGGAG133850.063.00576
TAGCTGG122150.061.9440237
AAGTGCT100550.061.617955
CCCGAGT33050.058.6812131
CTACTCG20700.057.405582
GCTACTC48550.057.2059481
TTTGGGA126000.056.7699625
GCACTTT111950.056.438591
TACTCGG21100.055.919243
GCGTAGC3101.2560104E-655.198814300-309
Produced by FastQC (version 0.11.5)