FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763334

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763334
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3417385
Sequences flagged as poor quality0
Sequence length35-251
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGTT2050.006271836151.03166245
CGAGTAG26100.082.489223
GCGACAT3350.001281632680.4203220-224
TTTCGTT10455.4569682E-1177.45711240-244
CCGAGTA27900.076.93352
AGTAGCT95600.076.6292955
CTACTCG24850.074.561692
GAGTAGC63200.074.016054
GTAGCTG100000.072.449356
TACTCGG26050.071.6278843
TTGGGAG148000.067.1055766
GCTGGGA198900.066.981379
GCTACTC57200.066.4267961
CCCGAGT33150.064.596151
GCACTTT117000.062.9976581
TTTGGGA130850.061.5197265
AAGTGCT101750.061.4832955
TAGCTGG121150.061.09327
GGAGGCT162800.060.924969
TCGGGAG34900.060.7265136
Produced by FastQC (version 0.11.5)