FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763335

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763335
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3355757
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG12450.045.5855373
CCGAGTA13400.045.1211932
CCGTACG705.2253643E-542.80991
AGTAGCT51300.042.268475
GCTGGGA98350.041.4923329
GAGTAGC32400.041.116224
TACTCGG10900.041.106223
TAGCTGG59600.040.323047
CTACTCG10550.040.287462
CCCGAGT16750.039.717361
GTAGCTG55450.038.7278866
TTGGGAG73300.036.276196
CTCGGGA14750.036.043365
GGAGGCT72800.035.0545239
GGGAGGC88950.033.237528
GCTACTC25650.033.1797561
CCCAAAG83600.031.4724431
GTGCTGG64700.030.984457
AAGTGCT66350.030.8802265
TCGGGAG16650.030.725746
Produced by FastQC (version 0.11.5)