FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763336

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763336
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3355757
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG10400.054.940583
CTACTCG11600.052.335642
TACTCGG11900.050.5160943
CCGAGTA11950.050.3047262
GCTACTC29600.048.904861
AGTAGCT51300.045.9403535
TTGGGAG81150.045.819066
GAGTAGC30200.045.329014
GCTGGGA104300.044.6043439
GGAGGCT84650.043.22189
GTAGCTG53900.043.156156
CGGAGGT19100.042.3604167
CCCGAGT15900.041.5875471
TAGCTGG60100.041.1787877
TCGGGAG17300.039.2024736
GGGAGGC108800.039.041288
CTCGGGA15550.038.6551635
AGGCGGA26400.036.5234
ACTCGGG16200.036.0052954
TGGGAGG107850.035.6893657
Produced by FastQC (version 0.11.5)