FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763337

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763337
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3674483
Sequences flagged as poor quality0
Sequence length125
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGAG2050.070.1646351
GAGTAGC24300.034.1586274
CGAGTAG9850.033.9530873
GCTGGGA74050.033.49919
TCGCGTT902.3441434E-433.1742865
CCGAGTA10750.032.290412
AGTAGCT41450.031.5495875
TACTCGG8650.031.0687583
CTCGCGT1352.2673412E-630.9639364
GGAGGCT61650.030.5878079
GCTACTC22750.030.5588821
CCCGAGT13900.029.3193931
GGGAGGC65800.029.2914778
TAGCTGG45700.029.2879167
GTAGCTG43150.028.9508066
CTACTCG8750.028.7273272
TTGGGAG59700.028.099396
CTCGGGA10550.027.7343315
CGTCGTA450.009542393526.43867150-51
GTCGAGA5500.026.1157512
Produced by FastQC (version 0.11.5)