FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763338

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763338
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3674483
Sequences flagged as poor quality0
Sequence length125
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATCG453.1736246E-452.882898
CGGAGGT12550.039.8201877
CCGAGTA9450.036.5372542
CGAGTAG9950.035.89783
GGGAGGC74550.034.155758
TTGGGAG61950.033.9961476
GGAGGCT66900.033.881819
GAGTAGC23850.033.696524
GCTGGGA80000.033.6136939
GCTACTC24650.033.3601381
CCCGAGT14000.032.773921
GGCGGAG18050.032.319525
GGAGGTT39800.032.2877968
AGGCGGA18450.031.6205484
GCGGAGG16750.030.9009746
AGTAGCT40900.030.5641295
TGGGAGG78350.030.297147
TAGCTGG48600.030.2362847
CTACTCG9150.029.9279122
CTCGGGA10400.029.7636415
Produced by FastQC (version 0.11.5)