FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763339

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763339
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3252988
Sequences flagged as poor quality0
Sequence length125
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCGA5350.098.605241
CGCGACT1100.076.296821
CGTCGAG11550.060.207021
GTCGAGA19200.036.1678662
CGTCCAG8100.031.779682
CCGTCGA1004.249741E-429.9737531
CGAGACT36900.029.0802251
GACTTGT50250.027.6944584
GCGACTT3600.026.6062452
CGACTTG4000.025.3856773
TCGAGAC29000.025.3341873
GTGATCA55800.025.2658949
ACGTCCA8800.025.20521
TGTGATC59200.023.8148128
ACTTGTG59150.023.627665
CTTGTGA61900.022.3916346
CGAGTAG9700.022.1682073
AGACTTG58200.022.0655773
GCTGGGA64800.021.389549
CCGAGTA9800.021.3800182
Produced by FastQC (version 0.11.5)