FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763340

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763340
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3252988
Sequences flagged as poor quality0
Sequence length125
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGAG1850.045.0899051
ACGTTCG1004.4273408E-429.764972
CCGAGTA9700.027.6169822
CGCGACT900.009466859526.4813731
CGAGTAG9900.025.8564383
GAGTAGC23600.024.7200584
AGTAGCT36350.023.7431815
CCCGAGT12600.023.6440811
GCTGGGA70150.023.3231229
GTCGAGA5050.022.41741
GCTACTC22550.021.9308031
GGCGTAG2454.2926513E-721.8876651
GTAGCTG40600.020.6621156
CGGAGGT10950.020.103397
TACTCGG9550.019.9472033
TAGCTGG43650.019.8998727
GGGAGGC60700.019.8970648
CCGATAG1500.00466986419.861031
GGAGGCT56550.019.6739089
CCCGTCG1550.00564466319.2203521
Produced by FastQC (version 0.11.5)