FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763341

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763341
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3367818
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG16050.063.2544823
CCGAGTA16800.060.1962742
GAGTAGC42800.057.623264
CTACTCG11750.057.039062
AGTAGCT69350.056.226225
TACTCGG13050.054.457023
CCCGAGT19700.053.8348961
GCTACTC30600.053.064571
GCTGGGA138700.052.3677679
GTAGCTG75450.051.8789866
TTGGGAG92600.049.979686
TAGCTGG85800.047.284847
GGAGGCT95750.044.732029
AAGTGCT76100.044.412375
GGGAGGC125250.042.412968
GCACTTT80600.042.0020261
TTTGGGA93200.041.069015
AAAGTGC79400.040.7633974
ACTTTGG82700.040.0778963
AGTGCTG82900.040.0409626
Produced by FastQC (version 0.11.5)