FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763344

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763344
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4066365
Sequences flagged as poor quality0
Sequence length35-251
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG22650.072.901783
CCGAGTA25850.065.636782
AGTAGCT88600.064.8597265
CTACTCG23500.064.1781852
GAGTAGC56050.063.6750564
TACTCGG24450.062.482963
GATCGTT5450.003578221762.076447240-244
TTGGGAG143250.061.3300136
GCTACTC54500.060.8751751
GTAGCTG94350.060.7460026
GCTGGGA191050.058.2974329
CCCGAGT30200.055.5749781
GGAGGCT153350.055.5526249
GCCGCAA5250.00559213455.458546220-224
TAGCTGG110700.052.8893437
GCACTTT118550.052.8434641
AAGTGCT107900.052.173875
TCGGGAG32400.051.744686
TTTGGGA136400.051.4713975
ACTTTGG122850.050.641833
Produced by FastQC (version 0.11.5)