FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763345

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763345
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3434789
Sequences flagged as poor quality0
Sequence length35-351
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTCG1601.6082673E-4135.53836190-199
TATAACG2900.0087602325135.04958330-339
CTTGCGC5756.236343E-496.37951320-329
CGAGTAG26750.079.9283453
AGTAGCT95350.076.259645
CCGAGTA28350.076.034342
GTAGCTG100800.072.5198756
GAGTAGC65750.070.624964
GCTGGGA190200.066.8388759
CCCGAGT33650.063.7268751
TTGGGAG127350.063.6366426
CGAGTAA3102.5192989E-563.16835330-339
TAGCTGG120250.062.1447037
CTACTCG20050.059.878942
AAGTGCT94950.059.0848925
TTTGGGA119450.058.3087435
TACTCGG21500.056.6904033
GCACTTT113250.056.1422121
GCTACTC49100.055.636081
GGAGGCT130550.054.8500989
Produced by FastQC (version 0.11.5)