FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763346

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763346
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3434789
Sequences flagged as poor quality0
Sequence length35-251
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGCG1150.006192005123.385765245
TACGCGA950.001454382795.07011230-234
CGAGTAG24850.079.845963
CTACTCG26800.079.429642
AGTAGCT94600.077.017165
TACTCGG27650.076.987863
GTAGCTG97200.074.2027366
GAGTAGC61950.074.132434
CCGAGTA26900.074.005292
GCTACTC59750.071.306991
GCTGGGA204100.069.87169
TTGGGAG146450.068.1770256
AAGTGCT103950.067.6880655
TCGGGAG35250.065.22186
CTCGGGA33900.063.565475
GGAGGCT163200.063.3129359
GCACTTT118100.063.2446021
TAGCTGG117550.063.14517
TTTGGGA128850.062.969415
CCCGAGT33250.062.091621
Produced by FastQC (version 0.11.5)