FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763349

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763349
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3330620
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG15100.068.01633
CCGAGTA16250.064.069052
GAGTAGC38300.058.3061944
TACTCGG11800.056.675783
CTACTCG11850.055.542672
AGTAGCT63400.055.0936975
CCCGAGT19150.054.7444461
GCTACTC29300.052.954511
GTAGCTG67000.050.697886
GCTGGGA125800.049.9357729
TTGGGAG87650.048.4590346
TAGCTGG73850.046.881447
GGAGGCT89950.045.0375149
GGGAGGC117650.043.1306048
ACTCGGG16450.042.105034
TTTGGGA86450.041.785515
AAGTGCT71150.041.7077525
TCGGGAG18750.040.9276586
GCACTTT74900.040.2306441
AGTGCTG75450.040.0528076
Produced by FastQC (version 0.11.5)