FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763350

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763350
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3330620
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG14000.065.896382
TACTCGG15100.063.066813
GCTACTC35450.062.1702581
CGAGTAG14500.059.5193023
CCGAGTA14950.057.7277572
TTGGGAG103850.056.3729066
GAGTAGC37300.056.327924
AGTAGCT61200.054.165475
GCTGGGA130550.052.7732059
GGAGGCT108050.051.703829
GTAGCTG63800.051.2889446
ACTCGGG19300.050.2675974
CCCAAAG98750.049.1003381
AAGTGCT77650.048.3622675
CCCGAGT19050.048.152951
CTCGGGA21150.048.1176575
GCACTTT86800.048.0369571
GGGAGGC142050.047.956348
TCGGGAG21950.047.4335146
TTTGGGA102400.046.8439875
Produced by FastQC (version 0.11.5)