FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763354

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763354
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3239697
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGAG1102.9491785E-629.9369351
CGAGTAG12250.025.6947083
CCGAGTA12700.025.5240962
AGTAGCT45700.024.4665345
GTAGCTG46250.023.7689026
TACTCGG12150.023.5862313
GTCGCCG4600.023.56229894
CGCCGTA2052.9076546E-822.9876194
GAGTAGC31000.022.5802944
GCTGGGA85900.022.1496499
CTACTCG11000.021.7812332
TTGGGAG63700.021.4614246
CCCGAGT17100.021.1834431
TAGCTGG54200.021.1492797
GGGAGGC85350.018.8797238
GCTACTC28050.018.1131041
CTCGGGA14550.018.0815945
GGAGGCT69250.018.0454149
CCAAAGT54150.017.9587822
TGGTCGC7350.017.95222794
Produced by FastQC (version 0.11.5)