FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763356

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763356
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3258045
Sequences flagged as poor quality0
Sequence length125
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTT1053.2570642E-739.6808365
CGGAGGT14050.038.110527
TACTCGG9450.035.2756423
CCGAGTA9200.034.29312
CTACTCG9250.034.1077352
GCTACTC25200.033.8200841
GGAGGCT62650.033.807089
TTGGGAG60750.033.591286
GGAGGTT36100.032.466678
CGAGTAG8850.032.2861823
GGGAGGC75250.032.178568
GCTGGGA77100.032.1009339
AGGCGGA18950.032.0412674
GAGTAGC22600.030.5540124
GGCGGAG19950.030.1335645
AGTAGCT36850.029.7202435
GCGGAGG18400.028.4540146
TGGGAGG79300.028.3594197
GTAGCTG38250.028.1530766
TCGGGAG13850.027.4921636
Produced by FastQC (version 0.11.5)