FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763357

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763357
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3077224
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA12000.045.3767432
CGAGTAG11550.044.4721683
CTACTCG9950.043.9008562
TACTCGG9850.043.0520633
TCGCGTT1002.1626329E-741.8062825
GAGTAGC27750.041.3233874
AGTAGCT45650.039.7718965
GCTACTC23650.039.0143051
GCTGGGA86400.037.523919
TTGGGAG60750.036.52486
CCCGAGT15600.036.486591
TAGCTGG49800.036.194177
GTAGCTG48300.035.8402636
CGTCGAG1251.2113542E-633.5523031
GGAGGCT65350.033.4986729
GGGAGGC76450.033.3814438
TCGGGAG14550.032.2990266
CTCGGGA13550.032.175635
CCCAAAG69700.030.3442381
AAGTGCT57300.029.9137425
Produced by FastQC (version 0.11.5)