FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763360

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763360
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3295765
Sequences flagged as poor quality0
Sequence length125
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG8850.043.7135163
TCGCGTT1053.260575E-739.675415
CCGAGTA10400.038.343092
CGGAGGT11600.036.4145287
GCTACTC23000.035.483581
CTACTCG9750.034.794922
GGCGGAG17200.033.9086385
CCCGAGT13200.033.8471451
GCTGGGA76250.033.3947539
TTGGGAG60050.033.189916
TACTCGG9400.032.924653
GAGTAGC23200.032.8373154
GGAGGCT66850.031.7717489
AGTAGCT38750.031.3307785
AGGCGGA18500.031.2065454
TCGGGAG13450.030.9635016
GTAGCTG41100.030.3984786
GCGGAGG17250.030.0057496
GGGAGGC76850.029.9599748
GGAGGTT35650.029.3715928
Produced by FastQC (version 0.11.5)