FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763361

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763361
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3012170
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG11950.051.4726523
CCGAGTA12700.050.883252
GAGTAGC32300.048.6234784
AGTAGCT53700.048.592695
GCTGGGA107350.047.046789
CTACTCG9450.046.8548662
GTAGCTG58300.044.7940446
TACTCGG9900.044.6378973
TAGCTGG64750.042.3531237
TTGGGAG68200.041.257326
GCTACTC23650.040.2871551
CCCGAGT16600.039.347671
GGAGGCT73900.036.545659
GGGAGGC92850.035.6859748
CCCAAAG80800.034.856771
AAGTGCT63200.034.7691885
AAAGTGC66150.033.8527684
GTGCTGG67000.033.8278547
CCAAGTA29300.033.4912222
AAGTAGC30350.033.448994
Produced by FastQC (version 0.11.5)