FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763362

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763362
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3012170
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG12850.053.7268833
TACTCGG12750.049.480313
CCGAGTA14100.049.3859632
GAGTAGC31800.048.8483774
GCTGGGA105550.048.248799
CTACTCG12900.047.9822272
AGTAGCT52150.047.815385
GTAGCTG54300.046.1267856
TTGGGAG76400.044.853886
TAGCTGG60250.042.5589757
CCCGAGT17100.042.148251
GCTACTC31200.041.237231
AAGTGCT63800.039.2707635
AAAGTGC64000.039.2436374
GGAGGCT87650.039.232589
CCCAAAG76400.039.216151
CTCGGGA18150.038.69135
GGGAGGC109150.037.6638958
TCGGGAG20450.036.9471866
ACTCGGG18450.036.774374
Produced by FastQC (version 0.11.5)