FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763364

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763364
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3880482
Sequences flagged as poor quality0
Sequence length125
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG11450.045.220983
CCGAGTA12800.043.7063062
TCGCGTT1455.820766E-1141.042695
CTACTCG10750.040.9684452
CCCGAGT16450.039.8275341
GAGTAGC26800.037.9741064
TACTCGG11400.037.58843
GCTGGGA94650.037.023329
AGTAGCT48600.036.6132935
CGGAGGT17200.035.973747
GGAGGCT72500.035.286719
TTGGGAG74450.035.0017786
GTAGCTG50000.034.9830786
TAGCTGG54000.034.7054337
GGGAGGC90300.033.8653878
GCTACTC27900.033.515991
CTCGGGA14450.032.124075
TGGGAGG95500.030.463957
GGAGGTT47550.030.029048
TCGGGAG15750.029.8419516
Produced by FastQC (version 0.11.5)