FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763365

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763365
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3215818
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG10400.055.1132433
CCGAGTA10950.051.9082832
GAGTAGC27650.049.446024
CGTCGAG1700.045.8083721
AGTAGCT48050.043.3633655
GTAGCTG49550.041.659516
CCCGAGT15800.041.3256651
GCTGGGA94550.040.078029
TAGCTGG59300.037.217537
TACTCGG9850.035.156843
CTACTCG9600.034.2782482
GGAGGCT67650.032.9755339
TTGGGAG68500.032.8268786
GCTACTC24350.032.2272151
GGGAGGC84500.031.6095248
TCGGGAG13400.029.7439276
GTGCTGG60200.029.5462957
AGGCGGA18000.029.1877864
GGAGGTT37350.028.6688488
GGCGGAG18200.028.5390055
Produced by FastQC (version 0.11.5)