FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763368

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763368
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4024595
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG12900.047.520053
CTACTCG13450.045.13382
CGAGTAG12200.044.880473
CCGAGTA12650.044.694812
GCTGGGA106950.039.2732479
GAGTAGC33400.038.8453834
GGAGGCT83950.038.4106339
CCCGAGT17600.037.2336851
TTGGGAG82100.036.2326246
CTCGGGA18200.035.6429565
TCGGGAG18600.034.8647426
TAGCTGG62150.034.7486887
AGTAGCT56050.034.720885
GGGAGGC104650.033.996558
GTAGCTG57950.033.36596
TCGCGTT1251.2767377E-633.3277975
CGGAGGT19550.032.8662267
ACTCGGG20050.032.0581744
GTGCTGG73750.030.009167
CCCAAAG88650.029.702951
Produced by FastQC (version 0.11.5)