FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763369

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763369
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3458117
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG13950.052.666363
CCGAGTA14850.049.571622
AGTAGCT58800.048.8576245
GAGTAGC34150.048.447634
GTAGCTG60900.046.886276
GCTGGGA118950.046.1592759
TAGCTGG68300.044.0708587
CCCGAGT17650.043.7975121
TTGGGAG85150.042.6154336
TACTCGG11600.041.1940423
CTACTCG11900.039.7314642
GCTACTC28000.038.9509281
GGAGGCT84700.038.4874279
GGGAGGC106900.038.0070958
AAGTGCT72250.037.0343555
CCCAAAG91700.035.941631
CTACTTG23950.035.4843832
GCACTTT73100.035.167731
AAAGTGC75950.035.153074
TTTGGGA88450.034.775555
Produced by FastQC (version 0.11.5)