FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763370

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763370
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3458117
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG12550.059.2878272
TACTCGG13050.057.472393
CGAGTAG14000.054.848013
CCGAGTA14400.053.324462
GCTACTC32950.052.9809881
GAGTAGC35200.051.238834
AGTAGCT57850.050.413635
TTGGGAG96250.049.1397866
GTAGCTG59700.048.232296
GCTGGGA120550.047.377369
CCCGAGT18800.045.3195951
GGAGGCT96700.045.158189
CTCGGGA17550.043.070695
TAGCTGG69800.042.702097
AAGTGCT74950.041.5322345
GGGAGGC125400.041.5123528
GCACTTT78100.041.2718621
TCGGGAG18750.041.248576
AAAGTGC76950.041.0757144
CCCAAAG93800.040.8428421
Produced by FastQC (version 0.11.5)