FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763371

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763371
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4026229
Sequences flagged as poor quality0
Sequence length125
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGAG3150.039.921431
CGAGTAG12150.037.340913
CCGAGTA12350.034.865722
ACGTCGA2004.9112714E-1132.935181
CTACTCG10100.031.9746252
GAGTAGC29850.031.3972844
TACTCGG10950.029.9844573
ATTCGCG800.005278736429.847424
GCTGGGA86950.029.7614779
CCCGAGT16450.029.4860381
AGTAGCT47500.028.6535225
TTGGGAG65600.028.293436
GTAGCTG49000.028.1661016
TAGCTGG53050.027.4735977
GGAGGCT67650.027.260189
TCGCGTT1107.6079153E-427.1340165
GCTACTC27550.026.735041
GGGAGGC78350.026.1947928
CTCGCGT1159.862555E-425.9542774
TGGGAGG84200.023.809637
Produced by FastQC (version 0.11.5)