FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763372

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763372
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4026229
Sequences flagged as poor quality0
Sequence length125
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG10000.038.685613
CCGAGTA11250.037.5614132
CTACTCG12800.036.2677572
TACTCGG13300.035.7992943
GGAGGCT76800.034.0851949
TTGGGAG75200.032.59526
GCTACTC29350.032.4704251
CGGAGGT16750.031.9670057
GAGTAGC29650.031.9160044
AGTAGCT48200.031.7310475
GCTGGGA89500.031.1762649
AGGCGGA20650.029.9743214
GGCGGAG20800.029.7555755
CTCGGGA15450.029.6592795
GGGAGGC91500.029.4544818
TAGCTGG51350.029.080887
GCGGAGG19650.028.7630736
GTAGCTG50200.028.4434196
CCCGAGT16200.028.3107811
TGGGAGG93050.027.9408327
Produced by FastQC (version 0.11.5)