FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763374

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763374
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3845834
Sequences flagged as poor quality0
Sequence length125
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACCG505.3248135E-447.6169623
CTACTCG11350.044.0509342
TACTCGG11400.042.8134963
CCGAGTA12150.039.1909182
CTCGGGA13850.037.8155755
GCTACTC27600.037.561861
CGGAGGT16300.036.1330537
GGAGGCT78350.035.459699
CGAGTAG11900.035.012473
TCGGGAG16600.034.7632756
GCTGGGA92200.034.0045289
GAGTAGC27950.033.647054
GGGAGGC93800.032.980538
TTGGGAG73900.032.925776
AGTAGCT46100.032.0175825
CCCGAGT17900.031.9539891
ACTCGGG17450.030.357524
TAGCTGG50150.029.894187
GTAGCTG48850.028.9847456
AGGCGGA21650.028.8670984
Produced by FastQC (version 0.11.5)