FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763375

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763375
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4043037
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG12950.042.866363
CCGAGTA13400.042.8572962
CTACTCG11350.040.5838472
TACTCGG12350.037.215793
GAGTAGC31150.037.1723634
GCTGGGA104450.036.7354979
GCTACTC28700.036.7275281
CCCGAGT18550.036.1605531
AGTAGCT51150.035.355535
GGAGGCT81950.034.1905949
TTGGGAG81200.034.1400836
TCGGGAG16400.033.7344636
TAGCTGG59650.033.708627
GTAGCTG54300.033.633596
GGGAGGC96900.033.458578
CTCGGGA16350.031.0285515
CCCAAAG89350.030.2303311
TGGGAGG101450.029.2019567
CGGAGGT17100.028.8746437
AAGTGCT74100.028.513165
Produced by FastQC (version 0.11.5)