FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763377

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763377
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3045159
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTT1152.6557245E-1046.721065
CTACTCG8150.042.5617142
TACTCGG8100.042.013963
GCTACTC22050.037.2121621
CTCGCGT2100.036.9572144
CTCGGGA10950.035.437845
GCTGGGA73000.035.3670659
CCGAGTA9550.035.0698132
CGAGTAG8750.034.7989353
AGTAGCT37500.034.7050935
CGGAGGT9950.031.6872797
TTGGGAG58200.031.584046
GAGTAGC24650.031.4848484
GGGAGGC67600.031.24028
TCGGGAG14700.031.1605826
GTAGCTG40900.030.8350546
CCCGAGT13050.030.7494411
TAGCTGG44900.030.3403997
GGAGGCT58700.029.2881499
ACTCGGG11650.029.2094974
Produced by FastQC (version 0.11.5)