FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763379

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763379
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3594321
Sequences flagged as poor quality0
Sequence length125
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG8400.039.900582
CGAGTAG10050.038.0304223
ACGCGAT650.001904678236.7505533
GCTGGGA88850.035.3507659
TACTCGG9700.035.0929873
CCGAGTA11800.034.4903342
AGTAGCT45100.034.4257745
CACGCGA700.002710501234.20052
CCCGAGT14700.033.8388141
CTCGGGA12100.032.572085
GAGTAGC27150.032.5534484
TCGGGAG12950.032.1551556
TAGCTGG50400.031.7500277
CGGAGGT11450.031.6917847
GCTACTC22950.031.5978181
GGAGGCT63450.031.2201489
TTGGGAG66000.030.8250986
GGGAGGC75500.030.570828
GTAGCTG49900.030.2799766
GTGCTGG62350.028.145437
Produced by FastQC (version 0.11.5)