FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763380

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763380
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3594321
Sequences flagged as poor quality0
Sequence length125
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG10850.048.2731632
CGAGTAG8800.045.3153083
TACTCGG11850.043.6972123
GCTACTC27800.043.0782051
CCGAGTA9950.042.470572
GGAGGCT79150.039.0105369
TTGGGAG71550.037.417476
GCTGGGA91700.036.591089
GAGTAGC25450.036.482924
AGTAGCT45850.035.1745345
GGGAGGC93400.034.778538
GTAGCTG47400.034.3909156
TAGCTGG52200.034.3057987
CGTACCG700.00278549734.010643
CCCGAGT13000.033.9152261
TCGGGAG16350.033.8404966
CGGAGGT16050.033.3609967
CTCGGGA16350.032.758445
ACTCGGG16950.032.305094
TCGCGTT953.2840835E-431.3216615
Produced by FastQC (version 0.11.5)