FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763381

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763381
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4107224
Sequences flagged as poor quality0
Sequence length125
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG11050.043.2256773
CCGAGTA12450.042.2867052
TACTCGG9350.039.590793
CTACTCG10200.036.95142
CCCGAGT17100.036.78531
CGGAGGT13800.032.7615857
AGTAGCT52750.032.5939035
GAGTAGC31100.032.058144
GTAGCTG55350.032.0279436
TTGGGAG78200.031.5697946
CACGCGA953.1840784E-431.4874062
GCTGGGA97250.031.3803799
GGGAGGC88350.030.3668968
TAGCTGG59400.029.9443687
AGCGTAC1602.610268E-729.7440726
TCGGGAG14250.029.6397066
GCTACTC27050.029.455441
CTCGGGA14050.029.3183235
GGAGGCT74800.028.8692469
CGTCGCA1254.7757283E-528.6554745
Produced by FastQC (version 0.11.5)