FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763382

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763382
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4107224
Sequences flagged as poor quality0
Sequence length125
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA11250.050.2588422
TACTCGG13100.048.6131673
CGAGTAG11300.047.9296653
CTACTCG12400.047.037672
TCGGGAG17150.041.974756
CTCGGGA16450.039.7966965
CCCGAGT14850.039.7080151
TCGCGTT1053.260393E-739.67615
GCTGGGA97850.038.2434169
GCTACTC31900.037.7163931
TTGGGAG82550.037.548066
GGGAGGC103500.037.19048
CGGAGGT17550.036.6111647
GGAGGCT88600.036.2599269
GAGTAGC30850.035.6909374
ACTCGGG18050.034.951844
AGTAGCT52850.032.7693825
TAGCTGG59600.032.641377
GGAGGTT48700.032.3730628
GTAGCTG53950.032.0899056
Produced by FastQC (version 0.11.5)