FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763384

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763384
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3629484
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG12350.050.1271443
CTACTCG12700.048.2769852
CGAGTAG9800.041.303753
GCTACTC30450.041.2957081
GGAGGCT83550.040.374759
CGGAGGT16800.039.6632167
TTGGGAG80550.039.220056
GCTGGGA99200.038.9829679
CCGAGTA11950.037.857532
CTCGGGA17950.037.8001175
TCGGGAG17950.037.785026
AGTAGCT46850.036.7147875
GTAGCTG48500.036.0649076
GGGAGGC101300.036.0610288
ACTCGGG18400.035.2626764
GAGTAGC27600.034.7234954
TAGCTGG55450.033.905087
CCCGAGT14450.033.818611
GGAGGTT48100.032.5304348
AGGCGGA24050.032.176214
Produced by FastQC (version 0.11.5)