FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763387

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763387
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4188366
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG11800.043.0056533
CTACTCG12700.042.391772
CGAGTAG15150.041.7716753
CCGAGTA15550.041.1619722
AGTAGCT59100.037.475195
CCCGAGT20900.037.2561571
GAGTAGC37150.036.9595344
GCTGGGA118950.036.6080939
TTGGGAG88200.035.949196
GTAGCTG63550.034.6351176
TAGCTGG67700.034.4451337
GCTACTC35200.034.3723871
GGGAGGC112050.033.128618
GGAGGCT93600.033.0489739
CGGAGGT16150.031.67797
CTCGGGA17750.031.2782175
TCGGGAG20000.031.2312816
ACTCGGG17800.030.1842174
TGGGAGG118150.029.5049157
AAGTGCT77750.028.7163095
Produced by FastQC (version 0.11.5)