FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763388

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763388
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4188366
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG14750.050.8421133
CTACTCG14800.049.865462
CGAGTAG13200.045.5399443
CCGAGTA13500.044.5274122
TCGGGAG19900.044.2452856
GCTACTC34250.043.8260881
CTCGGGA19200.041.845285
TTGGGAG91600.041.4365466
AGTAGCT57550.039.9168175
GCTGGGA119100.039.811179
CCCGAGT17600.038.5820121
GAGTAGC37750.038.469624
GGAGGCT105500.037.9508069
GGGAGGC125500.037.7335558
GTAGCTG59800.037.207356
ACTCGGG22300.034.9632264
CGGAGGT20350.034.204237
TAGCTGG69350.034.142477
TGGGAGG126350.033.430397
GGAGGTT57300.032.9126638
Produced by FastQC (version 0.11.5)