FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763390

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763390
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2666796
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA10600.039.444712
AGTAGCT39700.038.9330255
GCTACTC20150.038.0621761
TACTCGG8700.037.799983
CGAGTAG10550.036.960553
GTAGCTG40950.036.2531666
TTGGGAG61200.036.079396
GGAGGCT58100.035.4169169
CTACTCG8550.035.1656382
GCTGGGA72050.034.8845069
CCCGAGT13850.034.6522521
GAGTAGC25850.034.167254
TAGCTGG45950.033.2284937
GCACTTT49150.032.261711
ATCGCGA600.00410711931.41836494
CCCAAAG47700.031.0722851
TCGGGAG11850.030.9235296
AAAGTGC38600.030.9142844
GGGAGGC81650.030.6103448
CTCGGGA11900.030.0040155
Produced by FastQC (version 0.11.5)