FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763391

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763391
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3351940
Sequences flagged as poor quality0
Sequence length125
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGAG2600.046.066911
GCTACTC22900.034.5200081
CTACTCG8800.033.988412
GGAGGCT58950.028.5581159
TACTCGG9550.028.136183
GCTGGGA82350.027.9559529
CCGAGTA9950.027.0540922
GGGAGGC66200.026.4189938
TTGGGAG58150.025.9842936
CGAGTAG9800.025.5905273
AGTAGCT42550.025.5392635
GAGTAGC25150.025.4036054
TAGCTGG47800.025.0146877
CTCGGGA12450.024.938515
GTAGCTG44700.024.354016
TCGGGAG13300.024.1528846
TGGGAGG73700.023.165487
CCCAAAG72350.022.8456211
GTCGAGA4950.021.7525862
CCGGGTT9650.020.9593858
Produced by FastQC (version 0.11.5)