FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763393

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763393
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3026849
Sequences flagged as poor quality0
Sequence length125
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG6950.042.08483
CCGAGTA7650.035.965182
CTACTCG6550.035.6130522
CGTCGAG1805.638867E-1033.269041
GAGTAGC17950.032.2541244
CGGAGGT8300.030.8193867
TACTCGG7950.030.78443
CCCGAGT11750.030.0695481
ACGCGTA400.005369027629.7442938-39
AGTAGCT29900.029.5434595
GCTACTC16900.029.0562741
GCTGGGA60450.028.2435429
CCCGAAT5700.027.3156341
GGGAGGC52750.026.8403438
CTCGGGA9500.026.3873925
GGAGGCT48500.026.3712259
TAGCTGG37000.025.403237
GTAGCTG33300.025.1888566
TTGGGAG47300.025.1537326
TCGGGAG9800.024.2810526
Produced by FastQC (version 0.11.5)