FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763394

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763394
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3026849
Sequences flagged as poor quality0
Sequence length125
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATCG505.33802E-447.5927128
TCGCGTT951.4853686E-743.8542255
CGGAGGT10900.042.5714657
CTACTCG7450.039.1482852
GCTACTC19150.038.2620541
TACTCGG7750.037.633493
CTCGCGT1601.6916601E-1037.201474
AGGCGGA16000.033.853344
GCTGGGA61900.033.830049
GGCGGAG16350.032.761355
GGGAGGC61300.032.5113338
GGAGGCT53750.032.429459
GAGTAGC18200.031.7234544
GCGGAGG14500.031.5917156
TTGGGAG52800.030.984846
CGAGTAG6800.030.6365073
AGTAGCT31900.030.4112285
CTCGGGA10000.029.7582245
TAGCTGG35450.029.7034917
TCGGGAG10450.029.6031236
Produced by FastQC (version 0.11.5)