FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763395

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763395
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3302040
Sequences flagged as poor quality0
Sequence length125
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA10000.049.0626642
CGAGTAG10200.043.3083083
GAGTAGC25150.042.7209784
CCCGAGT14300.040.6358221
AGTAGCT41050.039.2600335
GTAGCTG41550.038.3702666
GCTGGGA79150.036.4520459
TAGCTGG48300.034.60917
CGTCGAG1953.6379788E-1133.793361
GCTACTC20150.031.8113481
TTGGGAG61300.031.151266
GGAGGCT60650.030.4061879
CGGAGGT11000.029.7441187
GGGAGGC67900.028.0356928
GGCGGAG15400.027.9069165
CCCAAAG72400.027.3881571
GCGGAGG13800.027.1576716
AGGCGGA15500.026.957094
TGGGAGG74950.026.906627
TACTCGG7400.026.6208483
Produced by FastQC (version 0.11.5)