FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763397

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763397
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3037217
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG11350.052.603913
CCGAGTA12100.051.423272
GAGTAGC29600.049.2142074
TACTCGG10400.045.3531653
AGTAGCT47850.044.6669465
CCCGAGT14650.044.1615871
CTACTCG9800.043.34552
GCTACTC23000.043.2353361
GCTGGGA95500.041.423739
TTGGGAG67850.041.2077526
GTAGCTG52500.040.791926
TAGCTGG55300.040.662837
GGGAGGC81800.038.3982628
GGAGGCT70600.038.001689
TCGGGAG14250.034.6492776
CTCGCGT1902.7284841E-1134.564594
TGGGAGG86100.034.476917
TCGCGTT1051.4505204E-534.1151355
GCACTTT60800.033.6962131
CTCGGGA13750.033.4328355
Produced by FastQC (version 0.11.5)