FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763398

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763398
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3037217
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG10800.056.7660832
TACTCGG11150.054.4503633
CGAGTAG9700.053.385463
GCTACTC26650.050.5302961
GAGTAGC26100.045.8385664
CCGAGTA11050.045.7859342
TTGGGAG77000.045.5857776
AGTAGCT44450.044.8559275
GCTGGGA97250.044.1069039
GGAGGCT81000.043.7745979
GTAGCTG45950.042.9853676
TCGGGAG16850.042.3691146
CCCGAGT13550.041.336071
CTCGGGA15900.041.1758355
GGGAGGC102800.040.8575638
ACTCGGG15350.039.939654
AAGTGCT60650.038.958895
TAGCTGG53450.038.734657
CCCAAAG76250.038.4473041
GCACTTT64900.038.377111
Produced by FastQC (version 0.11.5)