FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763401

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763401
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3578406
Sequences flagged as poor quality0
Sequence length125
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGAG2150.041.7883261
CGAGTAG11150.038.552883
CCGAGTA11300.036.0004162
CTACTCG9150.035.959912
GAGTAGC27200.034.6796884
TACTCGG9750.033.6788523
CGGAGGT12200.032.669747
AGTAGCT45100.032.563675
GCTGGGA86700.032.2484979
TTGGGAG60150.031.3512156
TAGCTGG52250.030.854167
CCCGAGT15950.030.7932381
GCTACTC24150.030.5063461
GTAGCTG50150.029.6551276
AGGCGGA17400.029.1646424
GGGAGGC73300.029.1354148
GGAGGCT66900.028.632589
GGCGGAG17750.027.9160885
ATGCGCG1505.1608204E-627.8608134
GGAGGTT37250.027.468378
Produced by FastQC (version 0.11.5)