FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763402

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763402
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3578406
Sequences flagged as poor quality0
Sequence length125
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG11750.053.69772
TACTCGG11550.053.082213
CGAGTAG11300.048.4622043
CCGAGTA12400.046.5626562
GCTACTC27750.042.4992641
GAGTAGC28150.041.4449354
CCCGAGT16350.040.0732081
CTCGGGA15750.038.5447125
TTGGGAG72300.038.2644966
CGGAGGT15600.038.1378947
TCGGGAG15650.038.0160526
GGAGGCT72400.037.7185869
GCTGGGA87600.037.354249
AGGCGGA21650.036.8417854
AGTAGCT46600.036.527955
GGGAGGC90500.036.1572538
ACTCGGG17550.035.9519544
GTAGCTG48050.035.2884676
GGCGGAG22300.034.4294475
TAGCTGG53450.034.2834367
Produced by FastQC (version 0.11.5)