FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763403

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763403
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2949623
Sequences flagged as poor quality0
Sequence length125
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTT701.3093086E-859.6897625
CGTCGAG1501.8189894E-1243.924151
CTACTCG6750.042.5379372
TACTCGG7000.038.37463
CCGAGTA8600.038.256322
CGAGTAG9200.035.0376783
CGGAGGT9200.033.6238827
GAGTAGC22450.033.23544
GCTACTC18400.032.878011
AGTAGCT34400.032.7946665
CCCGAGT12150.032.5364071
GGAGGCT50300.032.286959
GCTGGGA65500.032.2418069
TAGCTGG37550.032.1601757
GGGAGGC57700.032.0639428
TTGGGAG50050.031.3784986
CTCGGGA9200.030.4936815
GTAGCTG37900.029.8226836
CTCGCGT1306.2439874E-527.549594
TGGGAGG63450.027.3768547
Produced by FastQC (version 0.11.5)