FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763405

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763405
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5115616
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA20400.027.5310342
CGAGTAG19700.027.4122473
CTACTCG21150.027.2356472
AGTAGCT83650.026.681315
GCTGGGA163750.026.2924639
GTACGCG1400.00104152325.7130368
GTAGCTG87900.025.4731666
TACTCGG22650.025.1135713
GAGTAGC51000.024.8480384
GCTACTC53150.023.4375531
TTGGGAG117300.022.9554086
TAGCTGG102050.022.7877247
GGAGGCT134650.022.4571349
TCGGGAG28800.021.7489416
CCCGAGT30700.021.703841
CTCGGGA26400.021.2716945
GGGAGGC163000.020.0530138
GCTACTT47500.019.5165711
CAAGTAG45950.019.430583
GTGCTGG106150.019.2623717
Produced by FastQC (version 0.11.5)